Journal: Materials Today Bio

Article Title: 4D fabrication of scaffolds facilitates the construction of cholangiocyte monolayers from human and mouse liver derived organoids

doi: 10.1016/j.mtbio.2025.102757

Figure Lengend Snippet: PT-PLATMC@PDA scaffold supports the survival, proliferation and spreading of cholangiocytes to form a monolayer. Cell viability (A) and density (B) of cholangiocytes seeded on PT-PLATMC@PDA films in culture for 1 day, 3 days, and 7 days (n = 3). Fluorescence microscope images of mouse liver derived cholangiocyte organoid seeded on the PT-PLATMC@PDA film in culture for 7 days (C), 14 days (D), 21 days (E) and 35 days (F) stained with DAPI, ZO-1, and CK19. DAPI and ECAD staining of the 21-day cultured mouse cholangiocytes (G) and 37-day cultured human cholangiocytes (H) seeded SMP PT-PLATMC@PDA planar films.

Article Snippet: The samples were washed with PBS, mounted with 100 μl of anti-fade mounting medium with DAPI (Vector Laboratories Inc, USA), and analyzed under a confocal laser scanning microscope (Zeiss LSM880 with Ariyscan, Germany).

Techniques: Fluorescence, Microscopy, Derivative Assay, Staining, Cell Culture

Journal: Materials Today Bio

Article Title: 4D fabrication of scaffolds facilitates the construction of cholangiocyte monolayers from human and mouse liver derived organoids

doi: 10.1016/j.mtbio.2025.102757

Figure Lengend Snippet: Construction of a single-layer bile duct epithelium on PT-PLATMC@PDA tubular scaffolds. PT-PLATMC@PDA tubes seeded with (A) mouse derived cholangiocyte organoids cultured for 21 days and (B) human derived cholangiocyte organoids cultured for 37 days immuno-stained for DAPI, ZO-1and/or F Actin.

Article Snippet: The samples were washed with PBS, mounted with 100 μl of anti-fade mounting medium with DAPI (Vector Laboratories Inc, USA), and analyzed under a confocal laser scanning microscope (Zeiss LSM880 with Ariyscan, Germany).

Techniques: Derivative Assay, Cell Culture, Staining